Specifications and Use
Fluorescent Substrate: Dabcyl-PRAAAHomopheTSPK(5FAM)-NH2
Catalog Number: PEPDAB064m001, 1mg, 150 USD
Catalog Number: PEPDAB064m005, 5mg, 500 USD
This ADAM17 substrate is based on the TACEtide peptide used in Biochemical Journal, Cristina I. Caescu, Grace R. Jeschke, and Benjamin E. Turk (2009), 424(1), 79-88. and is the most sensitive substrate we have for ADAM17. See our Product Sheets for this ADAM17 substrate specificity profile. Typically, the ADAM17 substrate is dissolved in DMSO to make a stock solution of about 10mM concentration. When used for in vitro assays, the ADAM17 substrate is often used at about 10uM concentration. Remember to keep the DMSO concentration in the final reaction at 1% or below, to avoid DMSO effects on the reaction, and remember to have an equivalent percentage of DMSO in the background wells. For use with ADAMs, the buffer should consist of 25mM Tris, pH 8, 6 x 10-4 Brij detergent, and 10mM CaCl2. If used with ADAM17 or ADAM10, the CaCl2 is not required. For use with the MMPs, the buffer should contain 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM CaCl2, 5 µM ZnSO4, and 0.01% Brij-35. Excitation and emission wavelengths are 485 and 530 nm respectively.
Greater than 95% as assessed by HPLC and Mass Spectrometry.
1 mg/ml in water
Red lyophilized powder
The peptide powder is shipped at room temperature.
Upon receiving, the peptide should be stored at -70 oC. Avoid repeated freeze-thaw cycles. If dissolved in liquid (such as DMSO), aliquot into separate tubes to minimize the number of freeze-thaw cycles.
Samples are light sensitive and stable up to 6 months at -70oC.