MMP Substrate II (Fluorogenic) – PEPDAB061

Specifications and Use

Fluorescent Substrate: Dabcyl-GGCRPAHLRDSGK(5FAM)-NH2

Catalog Number:          PEPDAB061m001, 1mg, 80 USD

Catalog Number:          PEPDAB061m005, 5mg, 400 USD

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Substrate Specificity PEPDAB061

Use:

This mmp substrate can be used to assess activity of enzymes in the MMP family. The peptide sequence was described originally as a biosensor for MT1-MMP or MMP14 in “Simultaneous visualization of protumorigenic Src and MT1-MMP activities with fluorescence resonance energy transfer. Ouyang M, et al. Cancer Res. 2010 Mar 15;70(6):2204-12. doi: 10.1158/0008-5472.CAN-09-3698″. It demonstrates reasonably strong activity against MT1-MMP or MMP14 and MMP3, but has the highest activity against MMP9 with specificity constants, kcat/Km (M-1s-1), ranging from approximately 103 to 106. See also our Product Sheets for its substrate specificity profile. This substrate is not processed by ADAM family members. Typically, the peptide is dissolved in DMSO to make a stock solution of about 10mM concentration. When used for in vitro assays, the substrate is often used at about 10uM concentration. Remember to keep the DMSO concentration in the final reaction at 1% or below, to avoid DMSO effects on the reaction, and remember to have an equivalent percentage of DMSO in the background wells. For use with the MMPs, the buffer should contain 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM CaCl2, 5 µM ZnSO4, and 0.01% Brij-35. Excitation and emission wavelengths are 485 and 530 nm respectively.

Molecular Weight:

1962.2 g/mol

Purity:

Greater than 95% as assessed by HPLC and Mass Spectrometry.

Solubility:

1 mg/ml in water with 10% Formic acid

Appearance:

Red lyophilized powder

Shipping

The peptide powder is shipped at room temperature.

Storage:

Upon receiving, the peptide should be stored at -70 oC. Avoid repeated freeze-thaw cycles. If dissolved in liquid (such as DMSO), aliquot into separate tubes to minimize the number of freeze-thaw cycles.

Stability:

Samples are sensitive to light and stable up to 6 months at -70oC.

References:

Simultaneous visualization of protumorigenic Src and MT1-MMP activities with fluorescence resonance energy transfer. Ouyang M, et al. Cancer Res. 2010 Mar 15;70(6):2204-12. doi: 10.1158/0008-5472.CAN-09-3698.